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mixed primary antibodies against m1  (ATCC)


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  • 97

    Structured Review

    ATCC mixed primary antibodies against m1
    Mixed Primary Antibodies Against M1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 3566 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixed+primary+antibodies+against+m1/M-1/pm39014540-207-9-18
    Average 97 stars, based on 3566 article reviews
    mixed primary antibodies against m1 - by Bioz Stars, 2026-09
    97/100 stars

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    Incubation:

    Article Title: In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus
    Article Snippet: Briefly, 2-4 μm slides of the formalin-fixed paraffin-embedded specimen were deparaffinized, and sequentially incubated in 0.5% H2O2 in methanol for 30 minutes for inhibition of endogenous peroxidase, in citric acid buffer (pH 6,0) at 96°C for 25 minutes for unmasking of antigens, in 0,1 % Triton X-100 in Tris buffered saline (TBS) for 15 minutes for permeabilization, and SuperBlock TM blocking buffer (Thermo Fisher Scientific, USA) for 30 minutes for blocking excess binding sites. .. Afterwards, the slides were incubated with either single or mixed primary antibodies against M1 (monoclonal mouse anti-M1 antibody (ATCC, clone: M1Hb-64, 1:10) and NS1 (polyclonal anti-rabbit, 1:1000) at 4°C overnight, followed by incubation with AlexaFluor 488-conjugated donkey anti-mouse IgG (1:500, Dianova) and AlexaFluor 568-conjugated goat anti-rabbit IgG (1:500, Abcam) secondary antibodies at room temperature for 1 h. After further washing steps, sections were counterstained using 4’,6-diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) and mounted with glycerol-gelatin aqueous slide mounting medium (Sigma Aldrich). .. The labelled sections were analyzed using a motorized Axioplan 2 Imaging fluorescence microscope (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen) equipped with 25x/0.8 Plan-Neofluar water-immersion, 40x/1,2 Apochromat water-immersion, and 63x/1,2 Apochromat water-immersion objectives, an HBO 50 mercury-vapor short-arc lamp, AHF F31-000 (excitation 350/50, emission 460/50, for DAPI), AHF F41-054HQ (excitation 480/30, emission 527/30 HQ, for AF488), and AHF F41-007HQ (excitation 545/30, emission 610/75 HQ, for AF568) filter sets, and a monochrome 12 megapixel Axiocam 712 mono R2 CMOS camera.

    Article Title: In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus
    Article Snippet: Briefly, 2–4 μm slides of the formalin-fixed paraffin-embedded specimen were deparaffinized, and sequentially incubated in 0.5% H2O2 in methanol for 30 min for inhibition of endogenous peroxidase, in citric acid buffer (pH 6,0) at 96°C for 25 min for unmasking of antigens, in 0.1% Triton X-100 in Tris buffered saline (TBS) for 15 min for permeabilization, and SuperBlock TM blocking buffer (Thermo Fisher Scientific, USA) for 30 min for blocking excess binding sites. .. Afterwards, the slides were incubated with either single or mixed primary antibodies against M1 (monoclonal mouse anti-M1 antibody (ATCC, clone: M1Hb-64, 1:10) and NS1 (polyclonal anti-rabbit, 1:1000) at 4°C overnight, followed by incubation with AlexaFluor 488-conjugated donkey anti-mouse IgG (1:500, Dianova) and AlexaFluor 568-conjugated goat anti-rabbit IgG (1:500, Abcam) secondary antibodies at room temperature for 1 h. After further washing steps, sections were counterstained using 4,’6-diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) and mounted with glycerol-gelatin aqueous slide mounting medium (Sigma Aldrich). .. The labelled sections were analysed using a motorized Axioplan 2 Imaging fluorescence microscope (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen) equipped with 25×/0.8 Plan-Neofluar water-immersion, 40×/1,2 Apochromat water-immersion, and 63×/1,2 Apochromat water-immersion objectives, an HBO 50 mercury-vapour short-arc lamp, AHF F31–000 (excitation 350/50, emission 460/50, for DAPI), AHF F41-054HQ (excitation 480/30, emission 527/30 HQ, for AF488), and AHF F41-007HQ (excitation 545/30, emission 610/75 HQ, for AF568) filter sets, and a monochrome 12 megapixel Axiocam 712 mono R2 CMOS camera.

    Article Title: In turkeys, unlike chickens, the non-structural NS1 protein does not play a significant role in the replication and tissue tropism of the H7N1 avian influenza virus.
    Article Snippet: Briefly, 2–4 μm slides of the formalin-fixed paraffinembedded specimen were deparaffinized, and sequentially incubated in 0.5% H2O2 in methanol for 30 min for inhibition of endogenous peroxidase, in citric acid buffer (pH 6,0) at 96°C for 25 min for unmasking of antigens, in 0.1% Triton X-100 in Tris buffered saline (TBS) for 15 min for permeabilization, and SuperBlockTM blocking buffer (Thermo Fisher Scientific, USA) for 30 min for blocking excess binding sites. .. Afterwards, the slides were incubated with either single or mixed primary antibodies against M1 (monoclonal mouse anti-M1 antibody (ATCC, clone: M1Hb-64, 1:10) and NS1 (polyclonal anti-rabbit, 1:1000) at 4°C overnight, followed by incubation with AlexaFluor 488-conjugated donkey anti-mouse IgG (1:500, Dianova) and AlexaFluor 568-conjugated goat anti-rabbit IgG (1:500, Abcam) secondary antibodies at room temperature for 1 h. After further washing steps, sections were counterstained using 4,’6-diamidino-2-phenylindole (DAPI, 1:300, Invitrogen) and mounted with glycerolgelatin aqueous slide mounting medium (Sigma Aldrich). .. The labelled sections were analysed using a motorized Axioplan 2 Imaging fluorescence microscope (Carl Zeiss Microscopy Deutschland GmbH, Oberkochen) equipped with 25×/0.8 PlanNeofluar water-immersion, 40×/1,2 Apochromat water-immersion, and 63×/1,2 Apochromat waterimmersion objectives, an HBO 50 mercury-vapour short-arc lamp, AHF F31–000 (excitation 350/50, emission 460/50, for DAPI), AHF F41-054HQ (excitation 480/30, emission 527/30 HQ, for AF488), and AHF F41-007HQ (excitation 545/30, emission 610/75 HQ, for AF568) filter sets, and a monochrome 12 megapixel Axiocam 712 mono R2 CMOS camera.



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    97
    ATCC mixed primary antibodies against m1
    Mixed Primary Antibodies Against M1, supplied by ATCC, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mixed+primary+antibodies+against+m1/M-1/pm39014540-207-9-18
    Average 97 stars, based on 1 article reviews
    mixed primary antibodies against m1 - by Bioz Stars, 2026-09
    97/100 stars
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